1ANW

THE EFFECT OF METAL BINDING ON THE STRUCTURE OF ANNEXIN V AND IMPLICATIONS FOR MEMBRANE BINDING

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	83.900, 80.900, 71.400
Unit cell angles	90.00, 108.70, 90.00

Refinement procedure

Resolution	12.000 - 2.400
R-factor	0.181
RMSD bond length	0.017
RMSD bond angle	0.043
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	12.000 *
High resolution limit [Å]	2.400 *
Rmerge	0.067 *
Total number of observations	98492 *
Number of reflections	31600 *
Completeness [%]	83.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.4 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	PEG20000	5 (%)
3	1	drop		0.5 (mM)
4	1	drop	sodium maleate	50 (mM)
5	1	reservoir	PEG20000	20 (%)