1ANI

ALKALINE PHOSPHATASE (D153H, K328H)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-10-28
Detector	SAN DIEGO MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	194.600, 167.300, 76.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.176
Rwork	0.176
R-free	0.23300 *
RMSD bond length	0.019
RMSD bond angle	24.200 *
Data reduction software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.500
Rmerge	0.072
Total number of observations	118201 *
Number of reflections	39775
Completeness [%]	91.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	30 (mg/ml)
2	1	drop	ammonium sulfate	20 (%sat)
3	1	drop	Tris-HCl	100 (mM)
4	1	drop		10 (mM)
5	1	drop		0.01 (mM)
6	1	reservoir	ammonium sulfate	36-39 (%sat)