1AN9
D-AMINO ACID OXIDASE COMPLEX WITH O-AMINOBENZOATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.310, 92.580, 71.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26000 |
| Structure solution method | MR |
| Starting model (for MR) | 1AA8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.854 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.058 | |
| Total number of observations | 66408 * | |
| Number of reflections | 22424 | |
| Completeness [%] | 87.5 | 73.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.3 | 20 * | Setoyama, C., (1996) J. Biochem., 119, 1114. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 120 (mM) | |
| 3 | 1 | drop | sodium citrate | 60 (mM) | |
| 4 | 1 | drop | sodium benzoate | 0.2 (M) | |
| 5 | 1 | drop | PEG4000 | 5 (%) | |
| 6 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 8 | 1 | reservoir | PEG4000 | 30 (%) |
