1AN4
STRUCTURE AND FUNCTION OF THE B/HLH/Z DOMAIN OF USF
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 253 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06-01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 136.600, 54.700, 44.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.900 |
| Rwork | 0.236 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.050 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.076 | |
| Total number of observations | 32049 * | |
| Number of reflections | 6038 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 76.4 * | 49.3 |
| Redundancy | 2.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | pH 4.75, VAPOR DIFFUSION, HANGING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | GLYCEROL | ||
| 11 | 1 | 2 | KCL | ||
| 12 | 1 | 2 | MGCL2 | ||
| 13 | 1 | 2 | CD ACETATE | ||
| 14 | 1 | 2 | NA ACETATE | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | KCL | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | CD ACETATE | ||
| 7 | 1 | 1 | NA ACETATE | ||
| 8 | 1 | 2 | WATER | ||
| 9 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.4 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | |
| 4 | 1 | reservoir | PEG400 | 15 (%) | |
| 5 | 1 | reservoir | glycerol | 15 (%) | |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | 2.8 (mM) | ||
| 8 | 1 | reservoir | Cd(II) acetate | 1.4 (mM) | |
| 9 | 1 | reservoir | sodium acetate | 100 (mM) |
