1AMK
LEISHMANIA MEXICANA TRIOSE PHOSPHATE ISOMERASE
Experimental procedure
| Temperature [K] | 295 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-02-22 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.090, 52.940, 58.850 |
| Unit cell angles | 90.00, 118.13, 90.00 |
Refinement procedure
| Resolution | 22.000 - 1.830 |
| Rwork | 0.107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tim |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.808 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.049 * | 0.116 |
| Total number of observations | 123706 * | |
| Number of reflections | 22669 | |
| <I/σ(I)> | 20.8 | 6 |
| Completeness [%] | 89.2 | 84.3 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 100 MM ACETIC ACID/NAOH 4.5 20% PEG 6000 1 MM DTT, EDTA, 1 MM AZIDE, 5% ETHYLENE GLYCOL, 10 MM 2-PHOSPHOGLYCOLIC ACID. CRYSTAL WAS MOVED INTO 100 MM CITRATE PH 5.85 20 % PEG 6000, 1 MM DTT, 1 MM EDTA, 1 MM AZIDE BEFORE DATA COLLECTION. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 10 | 1 | reservoir | 1 (mM) | ||
| 11 | 1 | reservoir | acetic acid-NaOH | 100 (mM) | pH4.5 |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | reservoir | PEG6000 | 20 (%) | |
| 8 | 1 | reservoir | EDTA | 1 (mM) | |
| 9 | 1 | reservoir | dithiothreitol | 1 (mM) |
