1AM9
HUMAN SREBP-1A BOUND TO LDL RECEPTOR PROMOTER
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-10-24 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 94.630, 94.630, 459.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAX-DNA STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.770 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.480 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 * | |
| Number of reflections | 48155 | |
| <I/σ(I)> | 36 | |
| Completeness [%] | 86.9 * | 52.4 |
| Redundancy | 12 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | PROTEIN WAS CRYSTALLIZED FROM 20 % MPD, 100 MM KCL, 20 MM MGCL2, 100 MM HEPES, PH 5.6, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 3 | WATER | ||
| 11 | 1 | 3 | KCL | ||
| 12 | 1 | 3 | MGCL2 | ||
| 13 | 1 | 3 | MES | ||
| 14 | 1 | 3 | PEG 4000 | ||
| 15 | 1 | 3 | MPD | ||
| 2 | 1 | 1 | KCL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | TRIS-HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | KCL | ||
| 8 | 1 | 2 | MGCL2 | ||
| 9 | 1 | 2 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | reservoir | 150 (mM) | ||
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | MES | 100 (mM) | pH5.6 |
| 5 | 1 | reservoir | MPD | 10 (%) |
