1AM4
COMPLEX BETWEEN CDC42HS.GMPPNP AND P50 RHOGAP (H. SAPIENS)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.110, 78.120, 78.100 |
| Unit cell angles | 90.01, 90.00, 90.05 |
Refinement procedure
| Resolution | 12.000 - 2.700 |
| R-factor | 0.231 * |
| Rwork | 0.230 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1RGP 1mh1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.247 |
| Total number of observations | 246060 * | |
| Number of reflections | 49357 | |
| <I/σ(I)> | 26 | 5 |
| Completeness [%] | 97.6 | 96.5 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.9 | 4 * | pH 5.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 5 | 1 | reservoir | 3 (mM) |
