1ALU

HUMAN INTERLEUKIN-6

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	105
Detector technology	IMAGE PLATE
Collection date	1994-12
Detector	RIGAKU RAXIS II
Spacegroup name	P 31 2 1
Unit cell lengths	49.678, 49.678, 121.995
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.213 *
Rwork	0.213
R-free	0.27700
Structure solution method	MIR
RMSD bond length	0.017
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.031	0.129
Total number of observations	100519 *
Number of reflections	14002
<I/σ(I)>	15	5.8
Completeness [%]	97.6	88.5
Redundancy	7.2	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.3	4 *	PROTEIN AT 15 MG/ML WAS CRYSTALLIZED FROM 1.8M AMMONIUM SULFATE, 300 MM SODIUM POTASSIUM TARTRATE, IN 100MM PH 6.3 SODIUM CITRATE BUFFER.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	sodium potassium tartrate	300 (mM)
3	1	reservoir	sodium citrate	100 (mM)