1AL6
CHICKEN CITRATE SYNTHASE COMPLEX WITH N-HYDROXYAMIDO-COA AND OXALOACETATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-02-10 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.430, 78.480, 58.450 |
| Unit cell angles | 90.00, 78.89, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.850 |
| Rwork | 0.157 |
| Structure solution method | MOLECULAR SUBSTITUTION |
| Starting model (for MR) | 1csh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.200 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.047 | 0.155 |
| Number of reflections | 34332 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 87.0 | 78 |
| Redundancy | 1.7 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | CRYSTALLIZED FROM 1.02M NA CITRATE, PH 6.0 5 MM OXALOACETATE, PH 6 2 MM N-HYDROXYAMIDOCARBOXYMETHYLDETHIA COENZYME A. |
