1AKM
ORNITHINE TRANSCARBAMYLASE FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 32 |
| Unit cell lengths | 104.600, 104.600, 86.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.800 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT, SIRAS |
| Starting model (for MR) | 2at2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.126 | 0.300 |
| Number of reflections | 25858 | |
| <I/σ(I)> | 7.7 | 3.4 |
| Completeness [%] | 98.6 | 99.9 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 | 17 * | CRYSTALLIZED FROM 20% PEG 4000, 10% MPD, 1MM MGSO4, 50MM HEPES, PH 7.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | beta-octyl glucoside | 0.5 (%) | |
| 2 | 1 | 1 | PEG | 18 (%(w/v)) | |
| 3 | 1 | 1 | MPD | 10 (%(v/v)) | |
| 4 | 1 | 1 | 1 (mM) | ||
| 5 | 1 | 1 | HEPES | 20 (mM) |
