1AKL
ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-05-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.160, 176.690, 51.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.120 * |
| Data reduction software | WEIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.058 |
| Number of reflections | 44289 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 25 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (%) | |
| 2 | 1 | drop | PEG6000 | 6 (%(w/v)) | |
| 3 | 1 | drop | acetate | 50 (mM) | |
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 6 (%(w/v)) | |
| 6 | 1 | reservoir | acetate | 6 (%(w/v)) | |
| 7 | 1 | reservoir | 1 (mM) | ||
| 8 | 1 | drop | 5 (mM) |
