1AK9
SUBTILISIN MUTANT 8321
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1987-04-22 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.640, 79.450, 37.260 |
| Unit cell angles | 90.00, 114.53, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.148 * |
| Rwork | 0.148 |
| Structure solution method | DIFFERENCE MAP |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.030 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 17227 | |
| <I/σ(I)> | 16.9 | 1.4 |
| Completeness [%] | 78.6 | 55.9 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9 | Bryan, P.N., (1986) Proteins: Struct.,Funct., Genet., 1, 326. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | acetone | 55 (%) |
