1AK5
INOSINE MONOPHOSPHATE DEHYDROGENASE (IMPDH) FROM TRITRICHOMONAS FOETUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 157.250, 157.250, 157.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26500 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | PHASES |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.300 | |
| Rmerge | 0.057 | 0.250 * |
| Number of reflections | 153931 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging, sitting drop and liquid diffusion * | 7.4 | 2.2M AS, 2% PEG-550-MME, pH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-97 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.2 (M) |
