1AK0

P1 NUCLEASE IN COMPLEX WITH A SUBSTRATE ANALOG

Experimental procedure

Source details	ENRAF-NONIUS
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	41.980, 74.040, 102.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.207
Rwork	0.207
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.950
High resolution limit [Å]	1.800	1.800
Rmerge	0.068 *	0.247 *
Total number of observations	119199 *
Number of reflections	27854
Completeness [%]	93.2	89.9
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.3		PEG 6000 12 - 20% 25 MM NA ACETATE PH 5.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.8 (mM)
2	1	drop	DNA
3	1	drop	reservoir
4	1	reservoir	sodium acetate	25 (mM)
5	1	reservoir	PEG6000	12-20 (%(w/v))
6	1	reservoir	zinc chloride	2 (mM)