1AJZ

STRUCTURE OF DIHYDROPTEROATE PYROPHOSPHORYLASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	289
Detector technology	AREA DETECTOR
Collection date	1992-11
Detector	SIEMENS
Spacegroup name	C 1 21 1
Unit cell lengths	93.020, 60.200, 59.800
Unit cell angles	90.00, 114.50, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.185
Rwork	0.185
Structure solution method	MIR
RMSD bond length	0.017
RMSD bond angle	2.870
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	1.960	1.960
Rmerge	0.040 *
Total number of observations	64306 *
Number of reflections	19835
Completeness [%]	91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.4		PROTEIN WAS CRYSTALLIZED FROM 42% AMMONIUM SULFATE, 50MM TRIS-HCL, PH 8.4.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	42 (%sat)
2	1	1	Tris-HCl	50 (mM)