1AJ5
CALPAIN DOMAIN VI APO
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-24 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.500, 73.000, 156.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.30600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 20.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.190 |
| Total number of observations | 80668 * | |
| Number of reflections | 17172 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 97.7 | 94.2 * |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.8 | 38%SATURATED AMMONIUM SULFATE AND 25MM PIPES BUFFER, PH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate |
