1AIR

PECTATE LYASE C FROM ERWINIA CHRYSANTHEMI (EC16) TO A RESOLUTION OF 2.2 ANGSTROMS WITH 128 WATERS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1990-02
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	72.000, 83.000, 95.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.172
Rwork	0.172
R-free	0.22300
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.199 *
Data reduction software	SDMS (SOFTWARE)
Data scaling software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.200
Rmerge	0.175
Number of reflections	25685
Completeness [%]	95.1
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *	4 *	Kim, C-Y., (1989) J. Mol. Biol., 208, 365. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	HEPES	25 (mM)
3	1	drop		20 (mM)
4	1	drop	polygalacturonic acid	20 (%sat)
5	1	drop	PEG3350	10 (%(w/w))
6	1	reservoir	PEG3350		20ml