1AHW
A COMPLEX OF EXTRACELLULAR DOMAIN OF TISSUE FACTOR WITH AN INHIBITORY FAB (5G9)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 297 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-05 |
| Detector | SIEMENS |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 142.304, 73.312, 115.826 |
| Unit cell angles | 90.00, 90.89, 90.00 |
Refinement procedure
| Resolution | 7.000 - 3.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TISSUE FACTOR (PDB ENTRY 1TFH) AND FAB 5G9 (PDB ENTRY 1FGN) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 27.900 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | TFFC |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.135 * | 0.446 * |
| Total number of observations | 118598 * | |
| Number of reflections | 42650 | |
| <I/σ(I)> | 7.2 | 0.9 |
| Completeness [%] | 91.0 | 87 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | TF-5G9 CRYSTAL WERE GROWN IN 1.7-2.0M AMMONIUM SULFATE, 0.1M SODIUM CITRATE, PH 5.0-5.5, 0.2% 2-METHYL-2,4-PENTANE-DIOL (MPD), AND 2% PEG 600 AT AN EQUIMOLAR 5G9:TF RATIO. THE CRYSTALS GREW EXTREMELY SLOWLY, TAKING 6-9 MONTHS TO REACH A MAXIMAL SIZE OF 0.4 X 0.4 X 0.8 MM3., pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.7-2.0 (M) | |
| 2 | 1 | 1 | sodium citrate | 0.1 (M) | |
| 3 | 1 | 1 | MPD | 0.2 (%) | |
| 4 | 1 | 1 | PEG600 | 2 (%) |
