1AHS

CRYSTAL STRUCTURE OF THE TOP DOMAIN OF AFRICAN HORSE SICKNESS VIRUS VP7

Experimental procedure

Source type	ROTATING ANODE
Detector technology	IMAGE PLATE
Collection date	1993-06-15
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	157.200, 157.200, 57.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.213
Rwork	0.213
Structure solution method	MIR/MR
Starting model (for MR)	1bvp
RMSD bond length	0.017
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Rmerge	0.089
Number of reflections	29456
Completeness [%]	91.0
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	20 *
1	Vapor diffusion, sitting drop *	7.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop	urea	2.8 (M)
3	1	drop	protein	6-8 (mg/ml)
4	1	reservoir	ammonium sulfate	45-48 (%sat)
5	1	reservoir	Tris-HCl	10 (mM)