1AHE
ASPARTATE AMINOTRANSFERASE HEXAMUTANT
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-09-14 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.070, 77.980, 88.860 |
| Unit cell angles | 90.00, 118.63, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.203 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.270 |
| Data reduction software | MADNES |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Number of reflections | 42704 * |
| Completeness [%] | 88.6 * |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 20 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (mM) | |
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | DTT | 0.5 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.90-1.95 (M) | |
| 7 | 1 | reservoir | N-methylmorpholine | 200 (mM) | |
| 8 | 1 | reservoir | PEG400 | 2 (%) |
