1AH5
REDUCED FORM SELENOMETHIONINE-LABELLED HYDROXYMETHYLBILANE SYNTHASE DETERMINED BY MAD
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.470, 76.150, 50.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.462 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.134 |
| Number of reflections | 13486 | |
| <I/σ(I)> | 11.4 | 5.4 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | PROTEIN WAS CRYSTALLISED AT PH 5.3 IN SITTING DROPS OF 0.05ML WITH 6-7MG/ML OF PROTEIN, 0.3MM EDTA, 15MM DITHIOTHREITOL, 10%(W/V) PEG6000 AND 0.01% NAN3 IN 0.1M NAAC., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-7 (mg/ml) | |
| 2 | 1 | drop | EDTA | 0.3 (mM) | |
| 3 | 1 | drop | dithiothreitol | 15 (mM) | |
| 4 | 1 | drop | PEG6000 | 10 (%(w/v)) | |
| 5 | 1 | drop | 0.01 (%) | ||
| 6 | 1 | drop | sodium acetate | 0.1 (M) | |
| 7 | 1 | reservoir | dithiothreitol | 10-20mg |
