1AGR
COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | FUJI |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 164.000, 97.200, 110.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT, DENSITY MODIFICATION |
| Starting model (for MR) | 1gfi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AVGSYS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 * | |
| Number of reflections | 43341 | |
| <I/σ(I)> | 14.5 | 2.8 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | THE COMPLEX WAS CRYSTALLIZED IN HANGING DROPS USING PEG 10000 AS THE PRECIPITANT AND SODIUM CITRATE PH 5.3 AS THE BUFFER., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 2 | 1 | reservoir | PEG10000 | 14.4 (%(v/v)) | |
| 3 | 1 | reservoir | DTT | 5 (mM) | |
| 4 | 1 | reservoir | 0.140 (mM) | ||
| 5 | 1 | reservoir | 70 (mM) |
