1AGL
STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 294 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-05-01 |
| Detector | SDMS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 28.270, 28.270, 53.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DDF045 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.100 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.070 |
| Total number of observations | 11192 * |
| Number of reflections | 2058 |
| <I/σ(I)> | 5.5 |
| Completeness [%] | 93.0 |
| Redundancy | 6.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | pH 6.50, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | d(CGATCG) | 0.9 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 36 (mM) | |
| 3 | 1 | drop | 2.8 (mM) | ||
| 4 | 1 | drop | MPD | 5 (%) | |
| 5 | 1 | drop | spermine tetrahydrochloride | 5.7 (mM) | |
| 6 | 1 | drop | WP631 | 0.5 (mM) | |
| 7 | 1 | reservoir | 30 (%) |
