1AFR
STEAROYL-ACYL CARRIER PROTEIN DESATURASE FROM CASTOR SEEDS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.200, 147.800, 198.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.400 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28500 |
| Structure solution method | SIR + NCS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.075 |
| Total number of observations | 403539 * |
| Number of reflections | 79674 |
| Completeness [%] | 87.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 20 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 6-9 (mg/ml) | |
| 10 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 11 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
| 12 | 1 | reservoir | 2 (mM) | ||
| 13 | 1 | reservoir | 1 (mM) | ||
| 14 | 1 | reservoir | beta-octylglucoside | 0.2 (%) | |
| 15 | 1 | reservoir | PEG4000 | 12-15 (%) | |
| 2 | 1 | drop | cacodylate | 0.04 (M) | |
| 3 | 1 | drop | magnesium acetate | 100 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 37.5 (mM) | |
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | 0.5 (mM) | ||
| 7 | 1 | drop | beta-octylglucoside | 0.1 (%) | |
| 8 | 1 | drop | PEG4000 | 6-7.5 (%) | |
| 9 | 1 | reservoir | cacodylate | 0.08 (M) |
