1AEI

CRYSTAL STRUCTURE OF THE ANNEXIN XII HEXAMER

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1993-04
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	71.200, 179.200, 100.700
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.236
Rwork	0.236
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ANNEXIN I
RMSD bond length	0.018
RMSD bond angle	3.100
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	GLRF
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.800
Rmerge	0.068
Number of reflections	52044
<I/σ(I)>	4
Completeness [%]	86.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8	8 *	7 MG/ML ANX12, 65 W/V PEG 6000, 10 MM CACL2, PH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	annexin II	7 (mg/ml)
2	1	reservoir	PEG6000	6 (%(w/v))
3	1	reservoir		10 (mM)