1AE6

IGG-FAB FRAGMENT OF MOUSE MONOCLONAL ANTIBODY CTM01

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1995-02
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	54.300, 66.550, 61.350
Unit cell angles	90.00, 103.40, 90.00

Refinement procedure

Resolution	15.000 - 3.000
R-factor	0.21
Rwork	0.210
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1AD9 - STRUCTURE OF THE ENGINEERED HUMAN CONSTRUCT OF CTM01
RMSD bond length	0.006
RMSD bond angle	27.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.230
High resolution limit [Å]	3.000	3.000
Rmerge	0.138 *	0.252 *
Total number of observations	15707 *
Number of reflections	7862
<I/σ(I)>	5	2.1
Completeness [%]	91.1	88.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		20-25% PEG4000, BUFFERED AT PH 5.6 WITH 20MM BIS-TRIS.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	bis-Tris	20 (mM)
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	PEG4000	20-25 (%)
4	1	reservoir	Bis-Tris	20 (mM)