1ADQ

CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	1994-06
Detector	RIGAKU RAXIS II
Spacegroup name	C 1 2 1
Unit cell lengths	160.350, 81.950, 64.240
Unit cell angles	90.00, 98.27, 90.00

Refinement procedure

Resolution	8.000 - 3.150
R-factor	0.225
Rwork	0.225
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fc1 2ig2
RMSD bond length	0.005
RMSD bond angle	26.470 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.310
High resolution limit [Å]	3.150	3.150
Rmerge	0.113	0.265
Number of reflections	14109
<I/σ(I)>	5.3	2.8
Completeness [%]	98.3	98.8
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	17.5-21.5 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mg/ml)
2	1	drop		0.1 (%)
3	1	drop	Tris-HCl	20 (mM)
4	1	reservoir	PEG8000	17.5-22.5 (%(w/v))
5	1	reservoir		0.1 (%)
6	1	reservoir	Tris-HCl	100 (mM)