1AD0
FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY A5B7
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 1995-11 |
Spacegroup name | P 1 |
Unit cell lengths | 48.310, 66.270, 81.380 |
Unit cell angles | 69.88, 82.48, 71.34 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1clo |
RMSD bond length | 0.008 |
RMSD bond angle | 27.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.690 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.054 * | 0.179 * |
Total number of observations | 49216 * | |
Number of reflections | 29480 | |
<I/σ(I)> | 12.2 | 3.8 |
Completeness [%] | 94.9 | 94 |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | PEG6000/400 CACL2, BUFFERED AT PH6 WITH 40MM BIS-TRIS, pH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 18 (%) | |
2 | 1 | reservoir | 400 (mM) | ||
3 | 1 | reservoir | Bis-Tris | 40 (mM) | |
4 | 1 | drop | Bis-Tris | 20 (mM) | |
5 | 1 | drop | protain | 10 (mg/ml) |