1ABB

CONTROL OF PHOSPHORYLASE B CONFORMATION BY A MODIFIED COFACTOR: CRYSTALLOGRAPHIC STUDIES ON R-STATE GLYCOGEN PHOSPHORYLASE RECONSTITUTED WITH PYRIDOXAL 5'-DIPHOSPHATE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	119.000, 188.100, 88.100
Unit cell angles	90.00, 109.29, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.21
Rwork	0.210
RMSD bond length	0.020
RMSD bond angle	4.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.108 *
Total number of observations	107286 *
Number of reflections	78049 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	ammonium sulfate	1.2-1.4 (M)
3	1	2	IMP	6 (mM)
4	1	2	beta-glycerophosphate	10 (mM)
5	1	2	EDTA	0.5 (mM)
6	1	2	dithiothreitol	3 (mM)
7	1	2		0.02 (%)