1A9Z
UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GALACTOSE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 120 |
Detector technology | AREA DETECTOR |
Collection date | 1998-03 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.900, 83.900, 108.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.188 |
Rwork | 0.188 |
Structure solution method | FOURIER DIFFERENCE |
RMSD bond length | 0.007 |
RMSD bond angle | 15.048 * |
Data reduction software | SAINT |
Data scaling software | XCALIBRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.046 | 0.228 |
Total number of observations | 112562 * | |
Number of reflections | 32710 | 3288 * |
<I/σ(I)> | 24.6 | 2.9 |
Completeness [%] | 93.0 | 74 |
Redundancy | 3.4 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 | 4 * | CRYSTALLIZED FROM 18% PEG8000, 500MM NACL, 20MM UDP-GALACTOSE THEN SOAKED IN 25% PEG8000, 750MM NACL, 20MM UDP-GALACTOSE, 20% ETHYLENE GLYCOL, pH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 35 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 18-25 (%(w/v)) | |
3 | 1 | reservoir | 400-800 (mM) | ||
4 | 1 | reservoir | CHES | 50 (mM) |