1A92
OLIGOMERIZATION DOMAIN OF HEPATITIS DELTA ANTIGEN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.220, 85.300, 29.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.225 * |
| Rwork | 0.227 |
| R-free | 0.27000 * |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 16.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.180 * |
| Total number of observations | 221286 * | |
| Number of reflections | 28279 | |
| <I/σ(I)> | 51 | 7 |
| Completeness [%] | 94.0 | 35 * |
| Redundancy | 7.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.2 | 22 * | pH 5.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 100 (mM) | |
| 3 | 1 | drop | sodium citrate | 100 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) |
