1A8J

IMMUNOGLOBULIN LAMBDA LIGHT CHAIN DIMER (MCG) COMPLEX WITH ASPARTAME

Experimental procedure

Source type	SEALED TUBE
Source details	OTHER
Temperature [K]	286
Detector technology	DIFFRACTOMETER
Collection date	1988-11
Detector	SIEMENS
Spacegroup name	P 31 2 1
Unit cell lengths	72.300, 72.300, 185.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.22
Rwork	0.220
R-free	0.38000
Structure solution method	DIFFERENCE MAP
RMSD bond length	0.008
RMSD bond angle	31.100 *
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.820
High resolution limit [Å]	2.700	2.700
Number of reflections	10570
<I/σ(I)>	20	7
Completeness [%]	62.0	30

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.2		PROTEIN WAS CRYSTALLIZED IN FRESH 1.9M AMMONIUM SULFATE BUFFERED AT PH 6.2 WITH PHOSPHATE AND THE MOTHER LIQUOR WAS SATURATED WITH ASPARTAME.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	1.9 (M)
2	1	1	phosphate
3	1	1	aspartame