1A8H
METHIONYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 288 |
| Collection date | 1995-04 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.020, 82.470, 116.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.21 * |
| Rwork | 0.205 |
| R-free | 0.29100 * |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.070 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 * | 0.288 * |
| Total number of observations | 146275 * | |
| Number of reflections | 34121 * | |
| Completeness [%] | 89.6 * | 74.7 * |
| Redundancy | 4.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | PEG6000 | 5 (%) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 25 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
