1A7X
FKBP12-FK1012 COMPLEX
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 294 |
Detector technology | AREA DETECTOR |
Collection date | 1995-04-10 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 133.590, 40.510, 93.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.173 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fkf |
RMSD bond length | 0.010 |
RMSD bond angle | 2.800 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.046 * |
Number of reflections | 15896 |
<I/σ(I)> | 14 |
Completeness [%] | 91.0 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | drop contains equal volume of the reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | sodium formate | 5.1 (M) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) |