1A7T
METALLO-BETA-LACTAMASE WITH MES
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1996-02-05 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.270, 66.960, 69.480 |
Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.850 |
R-factor | 0.151 * |
Rwork | 0.151 |
Structure solution method | MIR |
RMSD bond length | 0.018 |
RMSD bond angle | 0.035 |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | PHASES |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.970 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.032 | 0.074 |
Number of reflections | 29508 | 3950 * |
<I/σ(I)> | 42.8 | 6.7 |
Completeness [%] | 91.3 | 73.9 |
Redundancy | 2.06 | 1.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.3 * | PROTEIN WAS CRYSTALLIZED FROM 20% PEG 4000, 100 MM POTASSIUM ACETATE, 100 MM MES, PH 6.1 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | enzyme | 10 (mg/ml) | |
2 | 1 | 1 | sodium cacodylate | 10 (mM) | |
3 | 1 | 1 | dithiothreitol | 2 (mM) | |
4 | 1 | 1 | sodium azide | 3 (mM) | |
5 | 1 | 2 | MES | 100 (mM) | |
6 | 1 | 2 | potassium acetate | 100 (mM) | |
7 | 1 | 2 | PEG4000 | 18-21 (%) |