1A7B
ENGINEERING A MISFOLDED FORM OF CD2
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.270, 80.030, 80.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.100 |
| R-factor | 0.2389 |
| Rwork | 0.239 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hng |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.272 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.080 |
| Rmerge | 0.059 | 0.295 |
| Number of reflections | 10589 | |
| <I/σ(I)> | 16.1 | 2.57 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 2.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 2M AMMONIUM SULFATE 2% PEG 400, 0.1M HEPES, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 2 | 1 | reservoir | PEG400 | 2 (%) | |
| 3 | 1 | reservoir | glycerol | 10 (%) | |
| 4 | 1 | reservoir | NaHEPES | 0.1 (M) |
