1A77

FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	58.830, 42.480, 62.560
Unit cell angles	90.00, 92.24, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.27500
RMSD bond length	0.008
RMSD bond angle	25.400 *
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.045 *
Total number of observations	49148 *
Number of reflections	20663 *
Completeness [%]	97.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%)
2	1	reservoir		5 (mM)
3	1	reservoir	HEPES/Na	100 (mM)