1A6T
FAB FRAGMENT OF MAB1-IA MONOCLONAL ANTIBODY TO HUMAN RHINOVIRUS 14 NIM-IA SITE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.170, 135.950, 81.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1for |
RMSD bond length | 0.010 |
RMSD bond angle | 1.870 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.042 | 0.150 |
Number of reflections | 29406 | |
<I/σ(I)> | 3700 | 581 |
Completeness [%] | 91.7 | 89.2 |
Redundancy | 2.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | SITTING DROP METHOD USING 18-22% PEG8000, 0.1 M SODIUM PHOSPHATE BUFFER (PH 6.0), AND 1% 2-METHYL-2,4-PENTANEDIOL WITH AN FAB1 CONCENTRATION OF 18 MG/ML., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Fab1 | 18 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 18-22 (%) | |
3 | 1 | reservoir | sodium phosphate | 0.1 (M) | |
4 | 1 | reservoir | MPD | 1 (%) |