1A6F

RNASE P PROTEIN FROM BACILLUS SUBTILIS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 64
Unit cell lengths	82.400, 82.400, 33.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.205
Rwork	0.205
R-free	0.31700
Structure solution method	MIRAS
RMSD bond length	0.011
RMSD bond angle	25.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLOMON
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.086 *	0.357 *
Total number of observations	15148 *
Number of reflections	4058
<I/σ(I)>	6	2.2
Completeness [%]	99.0	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		drop contains equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	mPEG550	23 (%)
4	1	reservoir		10 (mM)
5	1	reservoir	MES	100 (mM)