1A6E
THERMOSOME-MG-ADP-ALF3 COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 167.800, 167.800, 202.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.28500 |
| Structure solution method | DIFFERENCE FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 21.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.113 * | 0.458 * |
| Number of reflections | 22043 | |
| <I/σ(I)> | 6.2 | 1.6 |
| Completeness [%] | 92.0 | 61.4 |
| Redundancy | 3.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | 0.005ml of drop solution was mixed with 0.0025 ml of precipitant * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.3 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2 (M) | precipitant |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | precipitant |
