1A65
TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 273 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.390, 85.720, 143.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.230 |
R-factor | 0.16 * |
Rwork | 0.160 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoz |
RMSD bond length | 0.011 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.350 |
High resolution limit [Å] | 2.200 | 2.230 |
Rmerge | 0.100 | 0.270 |
Number of reflections | 152295 | |
<I/σ(I)> | 8.4 | 4.8 |
Completeness [%] | 99.6 | 98.5 |
Redundancy | 5.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 | 291 * | Ducros, V., (1997) Acta Crystallogr.,Sect.D, D53, 605. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | acetate | 10 (mM) | |
3 | 1 | reservoir | PEG8000 | 26-30 (%(v/v)) | |
4 | 1 | reservoir | acetate | 0.1 (M) |