1A58
CYCLOPHILIN FROM BRUGIA MALAYI
Experimental procedure
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.215, 58.215, 141.944 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.000 - 1.950 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.23400 * |
RMSD bond length | 0.008 |
RMSD bond angle | 26.020 * |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.950 * |
Rmerge | 0.092 * |
Total number of observations | 103228 * |
Number of reflections | 18055 * |
Completeness [%] | 96.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 50MM IMIDAZOLE AT PH6.5, 15% SAT. AMMONIUM SULFATE 0.02% SODIUM AZIDE, 0.56 MM PROTEIN CONC. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | imidazole | 50 (mM) | |
2 | 1 | drop | ammonium sulfate | 15 (%sat) | |
3 | 1 | drop | 0.02 (%(w/v)) | ||
4 | 1 | drop | bmCyp-1 | 0.56 (mM) | |
5 | 1 | reservoir | imidazole | 100 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 30 (%sat) | |
7 | 1 | reservoir | 0.02 (%(w/v)) |