1A4U

ALCOHOL DEHYDROGENASE FROM DROSOPHILA LEBANONENSIS

Experimental procedure

Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	274
Detector technology	IMAGE PLATE
Collection date	1997-02
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	65.495, 55.599, 70.070
Unit cell angles	90.00, 107.40, 90.00

Refinement procedure

Resolution	8.000 - 1.920
R-factor	0.205
Rwork	0.205
R-free	0.23800
Structure solution method	MR, SIR
Starting model (for MR)	2hsd
RMSD bond length	0.004
RMSD bond angle	24.960 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MAIN
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.980
High resolution limit [Å]	1.920	1.920
Rmerge	0.096	0.386
Total number of observations	124898 *
Number of reflections	36568
Completeness [%]	97.9	87
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	4 *	pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.3 (mg/ml)
2	1	reservoir	PEG2000	24 (%(w/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir	2-propanol	2 (%)