1A4O
14-3-3 PROTEIN ZETA ISOFORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Collection date | 1995-02 |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.900, 94.900, 236.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.31 |
| Rwork | 0.310 |
| R-free | 0.34500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.840 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.074 * |
| Number of reflections | 28237 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3500 | 20-24 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | 1 (mM) |
