1A2X

COMPLEX OF TROPONIN C WITH A 47 RESIDUE (1-47) FRAGMENT OF TROPONIN I

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06
Detector	MAR scanner 300 mm plate
Spacegroup name	P 32 2 1
Unit cell lengths	46.900, 46.900, 152.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.222
Rwork	0.222
R-free	0.32500
Structure solution method	SIR/MAD
RMSD bond length	0.010
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.300
Total number of observations	37214 *
Number of reflections	8870
<I/σ(I)>	9.2	2.3
Completeness [%]	96.3	94
Redundancy	4.5	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	16 *	Saijo, Y., (1997) Protein Sci., 6, 916. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	reservoir	sodium citrate	1.5 (M)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
4	1	reservoir	trehalose	15 (%)