1A2W
CRYSTAL STRUCTURE OF A 3D DOMAIN-SWAPPED DIMER OF BOVINE PANCREATIC RIBONUCLEASE A
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10 |
| Detector | RIGAKU |
| Spacegroup name | P 32 |
| Unit cell lengths | 57.000, 57.000, 81.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 * | |
| Total number of observations | 30428 * | |
| Number of reflections | 16601 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 96.0 | 92 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dimer | 7.5 (mg/ml) | |
| 10 | 1 | reservoir | 0.6 (M) | ||
| 11 | 1 | reservoir | PEG400 | 2 (%) | |
| 2 | 1 | drop | phosphate | 55 (mM) | |
| 3 | 1 | drop | sodium sulfate | 0.05 (M) | |
| 4 | 1 | drop | ADP | 5 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 0.8 (M) | |
| 6 | 1 | drop | 0.3 (M) | ||
| 7 | 1 | drop | PEG400 | 1 (%) | |
| 8 | 1 | reservoir | phosphate | 100 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 1.6 (M) |
