1A2B

HUMAN RHOA COMPLEXED WITH GTP ANALOGUE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1997-03
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 2
Unit cell lengths	62.020, 74.780, 50.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.195
Rwork	0.195
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5p21
RMSD bond length	0.010
RMSD bond angle	22.360 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.267
Total number of observations	61579 *
Number of reflections	8683
<I/σ(I)>	7.91	2.04
Completeness [%]	89.3	74.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	CRYSTALS WERE OBTAINED AT 277 K BY THE HANGING-DROP VAPOR DIFFUSION METHOD FROM SOLUTIONS CONTAINING 10 MG/ML(PROTEIN,GTPGAMMAS,MG2+ MIXTURE), 10% PEG 8000,7.5% 14-DIOXANE, 50 MM TRIS-HCL PH 8.5, EQUILIBRATED AGAINST 20% PEG 8000,15% 14-DIOXANE, 100 MM TRIS-HCL PH 8.5, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris/HCl	50 (mM)
3	1	drop	PEG8000	10 (%)
4	1	drop	1,4-dioxane	7.5 (%)
5	1	reservoir	Tris-HCl	100 (mM)
6	1	reservoir	PEG8000	20 (%)
7	1	reservoir	1,4-dioxane	15 (%)