1A1F
DSNR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GACC SITE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.000, 55.900, 128.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.23 * |
| Rwork | 0.225 |
| R-free | 0.27000 |
| Structure solution method | ISOMORPHOUS MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aay WITHOUT WATERS AND WITHOUT SIDE CHAINS FOR RESIDUES 18 - 24 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 * | |
| Total number of observations | 19390 * | |
| Number of reflections | 8185 | |
| <I/σ(I)> | 22.6 | 10.9 |
| Completeness [%] | 87.0 | 76 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 * | macroseeding * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | PRECIPITATING SOLUTION | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | NACL | ||
| 7 | 1 | 2 | TRIS | ||
| 8 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | zinc-finger-DNA complex | 0.5 (mM) | |
| 2 | 1 | drop | 250 (mM) | ||
| 3 | 1 | drop | 75 (mM) | ||
| 4 | 1 | drop | PEG3350 | 17.5 (%) | |
| 5 | 1 | reservoir | PEG3350 | 35 (%) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | MES | 75 (mM) |
