1A17

TETRATRICOPEPTIDE REPEATS OF PROTEIN PHOSPHATASE 5

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	90.060, 90.060, 104.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.201
Rwork	0.201
R-free	0.29800
Structure solution method	MIRAS
RMSD bond length	0.012 *
RMSD bond angle	1.754 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.82)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.073	0.133
Number of reflections	7832
<I/σ(I)>	24.4	3
Completeness [%]	96.8	97.6
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	4 *	1.8 M AMMONIUM SULFATE, 4% MPD, 100 MM HEPES PH 7.5, 2 MM DTT

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	1.8 (M)
2	1	reservoir	MPD	4 (%(v/v))
3	1	reservoir	HEPES	100 (mM)
4	1	reservoir	dithiothreitol	2 (mM)