1A16

AMINOPEPTIDASE P FROM E. COLI WITH THE INHIBITOR PRO-LEU

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	1996-08
Detector	RIGAKU RAXIS IV
Spacegroup name	P 64 2 2
Unit cell lengths	177.300, 177.300, 96.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.184
Rwork	0.184
R-free	0.22600
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.300	2.300 *
Rmerge	0.096 *	0.375 *
Total number of observations	549017 *
Number of reflections	36900 *
<I/σ(I)>	18	4.5
Completeness [%]	91.9 *	92 *
Redundancy	19.2	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3		drop contained 1:1 mixture of protein and reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.7 (mg/ml)
2	1	drop		5 (mM)
3	1	reservoir	PEG4000	30-33 (%)
4	1	reservoir	Tris-HCl	100 (mM)